4ILD
Crystal structure of truncated Bovine viral diarrhea virus 1 E2 envelope protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X25 |
| Synchrotron site | NSLS |
| Beamline | X25 |
| Temperature [K] | 195 |
| Detector technology | PILATUS 6M |
| Collection date | 2012-10-03 |
| Detector | S/N 60-0107 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.720, 54.450, 95.920 |
| Unit cell angles | 90.00, 92.23, 90.00 |
Refinement procedure
| Resolution | 47.970 - 3.270 |
| R-factor | 0.248 |
| Rwork | 0.246 |
| R-free | 0.28900 |
| Structure solution method | INITIAL SAD PLUS MULTIPLE HA SITE REFINEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.895 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MLPHARE |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.360 |
| High resolution limit [Å] | 3.270 | 3.270 |
| Number of reflections | 11169 | |
| <I/σ(I)> | 12.08 | 69.4 |
| Completeness [%] | 98.4 | 89.1 |
| Redundancy | 1.72 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.5 | 294 | 10% polyethylene glycol 3350, 0.1 M Bis-tris pH 5.5, 0.05 M cesium chloride, 0.04 M calcium acetate, 10% glycerol., VAPOR DIFFUSION, HANGING DROP, temperature 294K |
