4ILC
The GLIC pentameric ligand-gated ion channel in complex with sulfates
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-01-27 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9184 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 182.328, 132.100, 161.137 |
| Unit cell angles | 90.00, 103.02, 90.00 |
Refinement procedure
| Resolution | 34.530 - 2.990 |
| R-factor | 0.2165 |
| Rwork | 0.215 |
| R-free | 0.23630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hfi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.500 | 3.150 |
| High resolution limit [Å] | 2.990 | 2.990 |
| Rmerge | 0.085 | 0.543 |
| Number of reflections | 74893 | |
| <I/σ(I)> | 12.6 | 2.6 |
| Completeness [%] | 99.7 | 98.7 |
| Redundancy | 3.5 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 290 | 12-15% PEG 4K, 0.1M Na Acetate pH4, 0.2M NH4SO4, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
