4ILB
The pentameric ligand-gated ion channel GLIC A237F in complex with Rubidium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-01-01 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.8150 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 180.984, 132.412, 159.884 |
| Unit cell angles | 90.00, 102.09, 90.00 |
Refinement procedure
| Resolution | 36.370 - 3.150 |
| R-factor | 0.2062 |
| Rwork | 0.205 |
| R-free | 0.22560 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4hfi |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.130 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Refinement software | BUSTER (2.11.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.300 | 3.320 |
| High resolution limit [Å] | 3.150 | 3.150 |
| Rmerge | 0.136 | 0.400 |
| Number of reflections | 63790 | |
| <I/σ(I)> | 5.6 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 3.1 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4 | 290 | 12-15% PEG 4K, 0.1M Na Acetate pH4, 0.2M NaSCN, 0.2M RbCl, VAPOR DIFFUSION, HANGING DROP, temperature 290K |
