4IL8
Crystal structure of an H329A mutant of p. aeruginosa PMM/PGM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2011-10-22 |
Detector | NOIR-1 |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 70.962, 72.906, 93.030 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 57.380 - 1.800 |
R-factor | 0.18753 |
Rwork | 0.186 |
R-free | 0.21932 |
Structure solution method | refinement |
RMSD bond length | 0.015 |
RMSD bond angle | 1.503 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 57.380 | 1.860 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmerge | 0.056 | 0.491 |
Number of reflections | 45415 | |
<I/σ(I)> | 15.7 | 3.6 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 7.11 | 6.84 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | Na,K tartrate 1.2 M, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |