4IL3
Crystal Structure of S. mikatae Ste24p
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2012-08-19 |
Detector | MARMOSAIC 300 |
Wavelength(s) | 1.27 |
Spacegroup name | P 65 |
Unit cell lengths | 143.530, 143.530, 197.024 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.449 - 3.102 |
R-factor | 0.2706 |
Rwork | 0.270 |
R-free | 0.29260 |
Structure solution method | SAD, MOLECULAR REPLACEMENT |
Starting model (for MR) | 4aw6 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.778 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.150 |
High resolution limit [Å] | 3.100 | 8.400 | 3.100 |
Rmerge | 0.131 | 0.052 | 0.435 |
Number of reflections | 26917 | ||
<I/σ(I)> | 5 | 43.3 | 2.29 |
Completeness [%] | 64.8 | 99.9 | 17.5 |
Redundancy | 5.5 | 6.2 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 290 | 10% PEG 20,000, 20% PEG 550MME, 5 mM 1,6-hexanediol, 5 mM 1-butanol, 5 mM (RS)-1,2-propanediol, 5 mM 2-propanol, 5 mM 1,4-butanediol, 5 mM 1,3-propanediol, 100 mM MOPS / Na-HEPES pH 7.5, vapor diffusion, temperature 290K |