4IKY
Crystal structure of peptide transporter POT (E310Q mutant) in complex with sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-10-16 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.637, 93.536, 57.507 |
| Unit cell angles | 90.00, 112.38, 90.00 |
Refinement procedure
| Resolution | 32.639 - 2.100 |
| R-factor | 0.2155 |
| Rwork | 0.214 |
| R-free | 0.24850 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.733 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 5.700 | 2.100 |
| Rmerge | 0.084 | 0.052 | 0.295 |
| Number of reflections | 27920 | ||
| <I/σ(I)> | 9.2 | ||
| Completeness [%] | 92.8 | 98.4 | 85.3 |
| Redundancy | 2.7 | 3.5 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | 40% PEG 400, 100mM ADA-NaOH, 200mM Li2SO4, pH 6.5, lipidic cubic phase, temperature 293K |
