4IKW
Crystal structure of peptide transporter POT in complex with sulfate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-01-20 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.97944 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 52.505, 93.732, 57.265 |
| Unit cell angles | 90.00, 112.33, 90.00 |
Refinement procedure
| Resolution | 46.116 - 2.004 |
| R-factor | 0.1981 |
| Rwork | 0.196 |
| R-free | 0.23250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.019 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 2.030 |
| High resolution limit [Å] | 2.000 | 5.430 | 2.000 |
| Rmerge | 0.097 | 0.040 | 0.346 |
| Number of reflections | 33050 | ||
| <I/σ(I)> | 8.3 | ||
| Completeness [%] | 95.9 | 99.2 | 88.7 |
| Redundancy | 4.1 | 6 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 7 | 293 | 38% PEG 400, 300mM Li2SO4, 30mM SrCl2, pH 7.0, lipidic cubic phase, temperature 293K |
