4IKN
Crystal structure of adaptor protein complex 3 (AP-3) mu3A subunit C-terminal domain, in complex with a sorting peptide from TGN38
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-12-09 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 114.454, 44.301, 86.013 |
| Unit cell angles | 90.00, 127.84, 90.00 |
Refinement procedure
| Resolution | 67.930 - 1.851 |
| R-factor | 0.1937 |
| Rwork | 0.191 |
| R-free | 0.23750 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bxx |
| RMSD bond length | 0.026 |
| RMSD bond angle | 2.104 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (2.1.4) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.930 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Number of reflections | 28591 | |
| <I/σ(I)> | 3.2 | |
| Completeness [%] | 97.6 | 85.7 |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 294.15 | 0.1 M sodium acetate, 1.75 M sodium formate, pH 5.0, VAPOR DIFFUSION, HANGING DROP, temperature 294.15K |
