4IKG
Crystal structure of cell death-inducing DFFA-like effector c
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-11-24 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 |
| Unit cell lengths | 61.615, 61.615, 44.989 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.680 - 1.932 |
| R-factor | 0.2931 |
| Rwork | 0.292 |
| R-free | 0.30560 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.128 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | DM |
| Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 53.510 |
| High resolution limit [Å] | 1.932 |
| Number of reflections | 12963 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 291 | 0.2 M sodium acetate trihydrate, 0.1M TRIS hydrochloride pH8.5, 28% polyethylene glycol 4000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
