4IKG

Crystal structure of cell death-inducing DFFA-like effector c

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2009-11-24
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	1.5418
Spacegroup name	P 65
Unit cell lengths	61.615, 61.615, 44.989
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	26.680 - 1.932
R-factor	0.2931
Rwork	0.292
R-free	0.30560
Structure solution method	SAD
RMSD bond length	0.007
RMSD bond angle	1.128
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	DM
Refinement software	PHENIX ((phenix.refine: 1.6_289))

Data quality characteristics

	Overall
Low resolution limit [Å]	53.510
High resolution limit [Å]	1.932
Number of reflections	12963

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	0.2 M sodium acetate trihydrate, 0.1M TRIS hydrochloride pH8.5, 28% polyethylene glycol 4000, VAPOR DIFFUSION, HANGING DROP, temperature 291K