4IKD
Crystal structure of SNX11 PX domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Detector technology | CCD |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 40.120, 62.400, 66.060 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.290 - 1.600 |
| R-factor | 0.1885 |
| Rwork | 0.186 |
| R-free | 0.23740 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.751 |
| Data scaling software | SCALA (3.3.20) |
| Phasing software | MOLREP |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 45.362 | 34.291 | 1.690 |
| High resolution limit [Å] | 1.600 | 5.060 | 1.600 |
| Rmerge | 0.102 | 0.521 | |
| Rmeas | 0.120 | 0.598 | |
| Rpim | 0.061 | 0.288 | |
| Total number of observations | 2871 | 13827 | |
| Number of reflections | 22474 | ||
| <I/σ(I)> | 7.2 | 11.3 | 2.8 |
| Completeness [%] | 99.7 | 95 | 99.8 |
| Redundancy | 4.2 | 3.8 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 7.5 | 293 | 0.1M HEPES pH 7.5, 20% PEG 8000, vapor diffusion, temperature 293K |
