4IKD

Crystal structure of SNX11 PX domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Detector technology	CCD
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.9791
Spacegroup name	P 21 21 21
Unit cell lengths	40.120, 62.400, 66.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.290 - 1.600
R-factor	0.1885
Rwork	0.186
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.015
RMSD bond angle	1.751
Data scaling software	SCALA (3.3.20)
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	45.362	34.291	1.690
High resolution limit [Å]	1.600	5.060	1.600
Rmerge		0.102	0.521
Rmeas		0.120	0.598
Rpim		0.061	0.288
Total number of observations		2871	13827
Number of reflections	22474
<I/σ(I)>	7.2	11.3	2.8
Completeness [%]	99.7	95	99.8
Redundancy	4.2	3.8	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	0.1M HEPES pH 7.5, 20% PEG 8000, vapor diffusion, temperature 293K