4IKC

Crystal Structure of catalytic domain of PTPRQ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-10-28
Detector	ADSC QUANTUM 315
Wavelength(s)	1.
Spacegroup name	P 64
Unit cell lengths	77.580, 77.580, 85.240
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.394 - 1.560
R-factor	0.1483
Rwork	0.147
R-free	0.16540
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2gjt
RMSD bond length	0.013
RMSD bond angle	1.464
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.6.4_486))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	1.640
High resolution limit [Å]	1.560	1.560
Number of reflections	41238
Completeness [%]	99.8	100

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.6	291	0.1M sodium citrate, 0.2M magnesium acetate, 30% PEG4K, pH 5.6, VAPOR DIFFUSION, SITTING DROP, temperature 291K