4IJY

Crystal Structure of the ETEC Secreted Protein CofJ

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	CCD
Collection date	2008-02-22
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	0.9790
Spacegroup name	P 31 2 1
Unit cell lengths	143.489, 143.489, 59.290
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	20.000 - 2.600
R-factor	0.21377
Rwork	0.212
R-free	0.24082
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	1.188
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall
Low resolution limit [Å]	20.000
High resolution limit [Å]	2.600
Completeness [%]	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	100 mM BisTris (pH 6.5), 200 mM NaI and 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	100 mM BisTris (pH 6.5), 200 mM NaI and 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	100 mM BisTris (pH 6.5), 200 mM NaI and 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K