4IJY
Crystal Structure of the ETEC Secreted Protein CofJ
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-02-22 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.9790 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 143.489, 143.489, 59.290 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 20.000 - 2.600 |
R-factor | 0.21377 |
Rwork | 0.212 |
R-free | 0.24082 |
Structure solution method | MAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.188 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 20.000 |
High resolution limit [Å] | 2.600 |
Completeness [%] | 98.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM BisTris (pH 6.5), 200 mM NaI and 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM BisTris (pH 6.5), 200 mM NaI and 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 100 mM BisTris (pH 6.5), 200 mM NaI and 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |