4IJS
Crystal structure of nucleocapsid protein encoded by the prototypic member of orthobunyavirus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE BL-17A |
Synchrotron site | Photon Factory |
Beamline | BL-17A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-11-01 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 1.0000 |
Spacegroup name | I 4 2 2 |
Unit cell lengths | 106.140, 106.140, 485.187 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.746 - 3.200 |
R-factor | 0.2229 |
Rwork | 0.220 |
R-free | 0.27620 |
Structure solution method | SAD |
RMSD bond length | 0.011 |
RMSD bond angle | 1.548 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 3.200 |
Number of reflections | 23406 |
Completeness [%] | 99.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |