4IJ4
Crystal Structure of a Family GH19 chitinase from Bryum coronatum in complex with (GlcNAc)4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-07-04 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97932 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 74.546, 58.400, 48.066 |
| Unit cell angles | 90.00, 115.62, 90.00 |
Refinement procedure
| Resolution | 44.090 - 1.580 |
| R-factor | 0.154 |
| Rwork | 0.154 |
| R-free | 0.18100 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SnB |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.610 |
| High resolution limit [Å] | 1.580 | 1.580 |
| Rmerge | 0.119 | 0.281 |
| Number of reflections | 25456 | |
| <I/σ(I)> | 43.4 | 8.8 |
| Completeness [%] | 99.7 | 97.7 |
| Redundancy | 13.5 | 10 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.09M Tris-HCl(pH8.5), 1.35M di-Ammonium hydrogen phosphate, 0.001 M Cobalt(II) chroride, 0.01M Sodium acetate(pH4.6), 0.1M 1,6-Hexanediol, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
