4II7
Archaellum Assembly ATPase FlaI
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-25 |
| Detector | ADSC QUANTUM 315r |
| Spacegroup name | P 63 |
| Unit cell lengths | 130.018, 130.018, 311.772 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.920 - 3.590 |
| R-factor | 0.246 |
| Rwork | 0.243 |
| R-free | 0.30600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ihq |
| RMSD bond length | 0.018 |
| RMSD bond angle | 1.628 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.923 | 3.760 |
| High resolution limit [Å] | 3.590 | 3.590 |
| Number of reflections | 34740 | |
| Completeness [%] | 99.0 | 87.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 7 | 293 | 100mM HEPES pH 7.0, 22% Polyvinylpyrrolidone K 15, 10 mM CoCl2, 15% glycerol , VAPOR DIFFUSION, HANGING DROP, temperature 293K |
