4II1

Crystal structure of the zinc finger of ZGPAT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-04-08
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.28292
Spacegroup name	P 1 21 1
Unit cell lengths	55.170, 87.070, 76.570
Unit cell angles	90.00, 95.65, 90.00

Refinement procedure

Resolution	37.800 - 2.650
R-factor	0.2102
Rwork	0.208
R-free	0.24160
Structure solution method	SAD
RMSD bond length	0.009
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHELX
Refinement software	BUSTER-TNT (BUSTER 2.10.0)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.800		2.720
High resolution limit [Å]	2.650	11.850	2.650
Rmerge	0.073	0.037	0.946
Number of reflections	20804	224	1519
<I/σ(I)>	12.55	27.64	1.94
Completeness [%]	98.7	86.2	98.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	1.2 M sodium citrate, 5% MPD, 0.1 M sodium HEPES, 3 molar equivalents of H3K4me3 peptide., pH 7.5, vapor diffusion, sitting drop, temperature 291K