4IHU
Reduced form of disulfide bond oxdioreductase (DsbG) from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-05-18 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97602 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 71.030, 76.710, 86.960 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.700 - 1.896 |
| R-factor | 0.2024 |
| Rwork | 0.201 |
| R-free | 0.23130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3eu3 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.109 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SHELXS |
| Refinement software | PHENIX ((phenix.refine: 1.8_1069)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.700 | 1.943 |
| High resolution limit [Å] | 1.890 | 1.896 |
| Rmerge | 0.074 | 0.393 |
| Number of reflections | 38375 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 10.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 1.9 M ammonium sulfate, 9% isopropanol, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
