4IH4
Crystal structure of Arabidopsis DWARF14 orthologue, AtD14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 21-ID-D |
| Synchrotron site | APS |
| Beamline | 21-ID-D |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-03-21 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1.00 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 184.856, 44.629, 160.967 |
| Unit cell angles | 90.00, 121.07, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.500 |
| Rwork | 0.279 |
| R-free | 0.33630 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ih1 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Refinement software | CNS |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 30.000 | 30.000 | 3.620 |
| High resolution limit [Å] | 3.500 | 7.500 | 3.500 |
| Rmerge | 0.204 | 0.065 | 0.557 |
| Number of reflections | 12471 | ||
| <I/σ(I)> | 4 | ||
| Completeness [%] | 83.8 | 78.6 | 85.6 |
| Redundancy | 4.1 | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 298 | PEG4000, MgCl2, pH 8.5, vapor diffusion, temperature 298K |
