4IH1

Crystal structure of Karrikin Insensitive 2 (KAI2) from Arabidopsis thaliana

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2011-03-21
Detector	MAR scanner 300 mm plate
Wavelength(s)	1.00
Spacegroup name	P 1 21 1
Unit cell lengths	51.335, 56.013, 53.525
Unit cell angles	90.00, 116.15, 90.00

Refinement procedure

Resolution	30.000 - 1.550
R-factor	0.1797
Rwork	0.179
R-free	0.19270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wom
RMSD bond length	0.013
RMSD bond angle	1.272
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.000	30.000	1.610
High resolution limit [Å]	1.550	3.340	1.550
Rmerge	0.099	0.078	0.626
Number of reflections	39492
<I/σ(I)>	8.3
Completeness [%]	99.9	99.9	99.6
Redundancy	7.1	6.9	5.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	PEG400, ammonium sulfate, pH 7.5, vapor diffusion, temperature 298K