4IGI
Crystal structure of the Collagen VI alpha3 N5 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-10-06 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97780 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 37.730, 58.460, 39.160 |
| Unit cell angles | 90.00, 112.97, 90.00 |
Refinement procedure
| Resolution | 36.000 - 1.200 |
| R-factor | 0.121 |
| Rwork | 0.120 |
| R-free | 0.14780 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3gxb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.486 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.260 |
| High resolution limit [Å] | 1.200 | 1.200 |
| Rmerge | 0.045 | 0.174 |
| Number of reflections | 47672 | |
| <I/σ(I)> | 4.5 | |
| Completeness [%] | 97.0 | 93.8 |
| Redundancy | 2.4 | 2.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 294 | 0.1M sodium cacodylate, 1M tri-sodium citrate, pH 6.5, VAPOR DIFFUSION, SITTING DROP, temperature 294K |
