4IGB
Crystal structure of the N-terminal domain of the Streptococcus gordonii adhesin Sgo0707
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-11-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.979300 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 152.131, 158.115, 164.265 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 41.070 - 2.090 |
| R-factor | 0.181 |
| Rwork | 0.179 |
| R-free | 0.22500 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.103 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | Auto-Rickshaw |
| Refinement software | PHENIX ((phenix.refine: 1.6.4_486)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.450 | 2.200 |
| High resolution limit [Å] | 2.090 | 2.090 |
| Number of reflections | 114889 | |
| Completeness [%] | 98.4 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 291.15 | 20%(w/v) PEG4000, 0.1M sodium acetate, 0.2M ammonium sulphate, pH 5.0, VAPOR DIFFUSION, SITTING DROP, temperature 291.15K | ||
| 2 |
