4IG0
HIV-1 reverse transcriptase with bound fragment at the 507 site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-15 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.075 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 162.336, 73.351, 108.793 |
Unit cell angles | 90.00, 99.67, 90.00 |
Refinement procedure
Resolution | 43.141 - 2.500 |
R-factor | 0.2032 |
Rwork | 0.201 |
R-free | 0.24320 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 1.027 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (dev_1233) |
Refinement software | PHENIX ((phenix.refine: dev_1233)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.820 | 2.540 |
High resolution limit [Å] | 2.500 | 2.750 | 2.500 |
Rmerge | 0.063 | 0.293 | 0.609 |
Number of reflections | 43932 | ||
<I/σ(I)> | 33.56 | ||
Completeness [%] | 99.8 | 100 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | 11% PEG 8000, 4% PEG 400, 50 MM IMIDAZOLE, 10 MM SPERMINE, 15 MM MGSO4, 100 MM AMMONIUM SULFATE,AND 5 MM TRIS(2-CARBOXYETHYL)PHOSPHINE, PH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |