4IFY
HIV-1 reverse transcriptase with bound fragment at the Knuckles site
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-12-20 |
Detector | ADSC QUANTUM 270 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 161.946, 73.051, 108.755 |
Unit cell angles | 90.00, 100.47, 90.00 |
Refinement procedure
Resolution | 31.312 - 2.100 |
R-factor | 0.1869 |
Rwork | 0.186 |
R-free | 0.21260 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.190 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (dev_1233) |
Refinement software | PHENIX ((phenix.refine: dev_1233)) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.370 | 2.140 |
High resolution limit [Å] | 2.100 | 2.310 | 2.100 |
Rmerge | 0.077 | 0.352 | 0.629 |
Number of reflections | 73738 | ||
<I/σ(I)> | 17.4 | ||
Completeness [%] | 99.9 | 99.9 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.7 | 277 | 11% PEG 8000, 4% PEG 400, 50 MM IMIDAZOLE, 10 MM SPERMINE, 15 MM MGSO4, 100 MM AMMONIUM SULFATE, AND 5 MM TRIS(2-CARBOXYETHYL)PHOSPHINE, PH 6.7, VAPOR DIFFUSION, HANGING DROP, temperature 277K |