4IFE

Crystal Structure of the large terminase subunit gp2 of bacterial virus Sf6 complexed with ATP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	298
Detector technology	CCD
Collection date	2012-04-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.03319
Spacegroup name	P 21 21 21
Unit cell lengths	58.176, 63.396, 151.212
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.904 - 3.050
R-factor	0.2118
Rwork	0.208
R-free	0.27920
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	4idh
RMSD bond length	0.009
RMSD bond angle	1.299
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	100.000	3.100
High resolution limit [Å]	3.050	3.050
Rmerge	0.126	0.235
Number of reflections	9962
<I/σ(I)>	21.02	5.08
Completeness [%]	87.4	64.7
Redundancy	2.8	1.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100mM Tris, 15% ethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K