4IEI
Crystal Structure of the large terminase subunit gp2 of bacterial virus Sf6 complexed with ADP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2012-11-19 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.00318 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.468, 63.213, 149.779 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.783 - 2.090 |
R-factor | 0.1769 |
Rwork | 0.175 |
R-free | 0.21090 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4idh |
RMSD bond length | 0.008 |
RMSD bond angle | 1.162 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.8.1_1168)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.120 | 2.140 |
High resolution limit [Å] | 2.090 | 2.090 |
Rmerge | 0.064 | 0.740 |
Number of reflections | 32795 | |
<I/σ(I)> | 13.7 | 1.6 |
Completeness [%] | 98.8 | 91.7 |
Redundancy | 3.7 | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100mM Tris, 15% ethanol , pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |