4IEG
Structure and interactions of the RNA-dependent RNA polymerase from bacteriophage phi12 (P1 crystal form)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4C |
Synchrotron site | NSLS |
Beamline | X4C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-03 |
Detector | MAR CCD 165 mm |
Wavelength(s) | 0.979 |
Spacegroup name | P 1 |
Unit cell lengths | 87.874, 94.470, 96.481 |
Unit cell angles | 75.16, 63.11, 83.79 |
Refinement procedure
Resolution | 50.000 - 2.100 |
R-factor | 0.22025 |
Rwork | 0.217 |
R-free | 0.27836 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4gzk |
RMSD bond length | 0.008 |
RMSD bond angle | 1.100 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rmerge | 0.048 | 0.202 |
Number of reflections | 144893 | |
<I/σ(I)> | 17.8 | 2.9 |
Completeness [%] | 92.6 | 87.1 |
Redundancy | 1.9 | 1.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.2 M Mg formate 20% PEG 3350, pH 8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |