4IEE
Crystal Structure of the large terminase subunit gp2 of bacterial virus Sf6 complexed with ATP-r-S
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2012-04-17 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 1.03319 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.101, 63.142, 149.491 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.748 - 1.890 |
R-factor | 0.1676 |
Rwork | 0.166 |
R-free | 0.20760 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 4idh |
RMSD bond length | 0.007 |
RMSD bond angle | 1.036 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 100.000 | 1.920 |
High resolution limit [Å] | 1.890 | 1.890 |
Rmerge | 0.045 | 0.363 |
Number of reflections | 43101 | |
<I/σ(I)> | 29.92 | 3.29 |
Completeness [%] | 97.5 | 91.7 |
Redundancy | 3.4 | 3.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 100mM Tris, 15% ethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K |