4IED
Crystal Structure of FUS-1 (OXA-85), a Class D beta-lactamase from Fusobacterium nucleatum subsp. polymorphum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-11-08 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.934 |
| Spacegroup name | P 1 |
| Unit cell lengths | 43.945, 65.670, 91.698 |
| Unit cell angles | 84.59, 82.91, 70.12 |
Refinement procedure
| Resolution | 37.590 - 1.500 |
| R-factor | 0.14961 |
| Rwork | 0.146 |
| R-free | 0.18428 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.131 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.590 | 1.580 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.062 | 0.246 |
| Number of reflections | 146121 | |
| <I/σ(I)> | 17.7 | 6.1 |
| Completeness [%] | 95.5 | 94 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | FUS-1 15mg/mL in 10 mM Tris pH=8.0, 0.1M NaCl, 4.0 M Na/NH4 formate, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
