4IDW

Polycrystalline T6 Bovine Insulin: Anisotropic Lattice Evolution and Novel Structure Refinement Strategy

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID13
Synchrotron site	ESRF
Beamline	ID13
Temperature [K]	295
Detector technology	CCD
Collection date	2006-11-07
Detector	Frelon4M 2k x 2k CCD camera
Wavelength(s)	1.29967, 0.53395
Spacegroup name	H 3
Unit cell lengths	82.607, 82.607, 33.632
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	? - ?
Data reduction software	FIT2D
Data scaling software	ID31sum
Phasing software	MOLREP
Refinement software	GSAS

Data quality characteristics

	Overall
Low resolution limit [Å]	18.200
High resolution limit [Å]	2.700
Number of reflections	2140
Completeness [%]	70.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Batch	6	295	polycrystalline samples of T6 hexameric insulin (Zn2[(AB)2]3, the pH was adjusted by mixing different ratios of 0.2M solutions of sodium monobasic monohydrate phosphate and potassium phosphate., Batch, temperature 295K
1	Batch	6	295	polycrystalline samples of T6 hexameric insulin (Zn2[(AB)2]3, the pH was adjusted by mixing different ratios of 0.2M solutions of sodium monobasic monohydrate phosphate and potassium phosphate., Batch, temperature 295K