4IDI
Crystal Structure of Rurm1-related protein from Plasmodium Yoelii, PY06420
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-11-28 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.97942 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.590, 72.720, 107.510 |
| Unit cell angles | 90.00, 92.64, 90.00 |
Refinement procedure
| Resolution | 43.200 - 1.900 |
| R-factor | 0.2284 |
| Rwork | 0.227 |
| R-free | 0.25210 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.070 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | BALBES |
| Refinement software | BUSTER-TNT |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.970 |
| High resolution limit [Å] | 1.900 | 4.090 | 1.900 |
| Rmerge | 0.142 | 0.098 | 0.510 |
| Number of reflections | 39268 | ||
| <I/σ(I)> | 5.2 | 2.14 | |
| Completeness [%] | 99.4 | 100 | 95.3 |
| Redundancy | 6.7 | 7.5 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.8 | 293 | 25 % PEG 3350, 0.2 M NaCl, 0.1 M Na Cacodylate, 0.1 ug/ml chymotrypsin, pH 5.8, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
