4IDH

Crystal Structure of the large terminase subunit gp2 of bacterial virus Sf6

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-B
Synchrotron site	APS
Beamline	23-ID-B
Temperature [K]	298
Detector technology	CCD
Collection date	2011-10-21
Detector	MARMOSAIC 325 mm CCD
Wavelength(s)	1.03304
Spacegroup name	P 21 21 21
Unit cell lengths	57.790, 63.440, 150.730
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.859 - 1.690
R-factor	0.1828
Rwork	0.181
R-free	0.21570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	nuclease domain of gp2 of bacterial virus sf6 and ATPase domain of gp17 of T4 bacteriophage
RMSD bond length	0.007
RMSD bond angle	1.020
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASES
Refinement software	PHENIX ((phenix.refine: 1.8.1_1168))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.720	1.780
High resolution limit [Å]	1.690	1.690
Rmerge	0.115	0.549
Number of reflections	62415
<I/σ(I)>	7.1	2.3
Completeness [%]	99.3	100
Redundancy	4.9	4.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100mM Tris, 15% ethanol, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293K