4ID6
Crystal Structure of Staphylococcal nuclease mutant V23I/I72L
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2012-04-12 |
| Detector | RIGAKU SATURN 92 |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 41 |
| Unit cell lengths | 48.300, 48.300, 63.900 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.600 - 1.898 |
| R-factor | 0.19012 |
| Rwork | 0.188 |
| R-free | 0.24150 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PBD ENTRY 1EY0 |
| RMSD bond length | 0.024 |
| RMSD bond angle | 2.067 |
| Data reduction software | d*TREK |
| Data scaling software | d*TREK |
| Phasing software | CCP4 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 21.620 | 2.580 | 1.970 |
| High resolution limit [Å] | 1.898 | 2.390 | 1.898 |
| Rmerge | 0.048 | 0.036 | 0.048 |
| Number of reflections | 11415 | ||
| <I/σ(I)> | 10.2 | 4.8 | 1.3 |
| Completeness [%] | 98.1 | 97.6 | 98.1 |
| Redundancy | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
