4ID6
Crystal Structure of Staphylococcal nuclease mutant V23I/I72L
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 298 |
Detector technology | CCD |
Collection date | 2012-04-12 |
Detector | RIGAKU SATURN 92 |
Wavelength(s) | 1.5418 |
Spacegroup name | P 41 |
Unit cell lengths | 48.300, 48.300, 63.900 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 21.600 - 1.898 |
R-factor | 0.19012 |
Rwork | 0.188 |
R-free | 0.24150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PBD ENTRY 1EY0 |
RMSD bond length | 0.024 |
RMSD bond angle | 2.067 |
Data reduction software | d*TREK |
Data scaling software | d*TREK |
Phasing software | CCP4 |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 21.620 | 2.580 | 1.970 |
High resolution limit [Å] | 1.898 | 2.390 | 1.898 |
Rmerge | 0.048 | 0.036 | 0.048 |
Number of reflections | 11415 | ||
<I/σ(I)> | 10.2 | 4.8 | 1.3 |
Completeness [%] | 98.1 | 97.6 | 98.1 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277 | pH 8, VAPOR DIFFUSION, HANGING DROP, temperature 277K |