4ID3
Crystal Structure of the BRCT domain of S. Cerevisiae Rev1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2011-06-16 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.003, 82.491, 32.705 |
| Unit cell angles | 90.00, 91.49, 90.00 |
Refinement procedure
| Resolution | 17.448 - 1.967 |
| R-factor | 0.192 |
| Rwork | 0.191 |
| R-free | 0.23320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2ebw |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.096 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: dev_1051)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 17.450 | 2.070 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.027 | 0.255 |
| Number of reflections | 11413 | |
| <I/σ(I)> | 19.5 | 4.7 |
| Completeness [%] | 94.1 | 85.1 |
| Redundancy | 2.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 291 | 20% PEG 2000, 0.1M HEPES , pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
