4ICN
Dihydrodipicolinate synthase from shewanella benthica
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-26 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.95369 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.090, 84.030, 143.720 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.330 - 2.500 |
| R-factor | 0.20162 |
| Rwork | 0.199 |
| R-free | 0.25008 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDB ID 3G0S |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.543 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 72.540 | 2.640 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 31280 | |
| Completeness [%] | 99.7 | 98.2 |
| Redundancy | 3.7 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 281 | 0.2m AMMONIUM SULFATE, 25%(W/V) PEG 3350, 0.1 BIS-TRIS (PH 5.5), VAPOR DIFFUSION, HANGING DROP, temperature 281K |
