4ICD
REGULATION OF ISOCITRATE DEHYDROGENASE BY PHOSPHORYLATION INVOLVES NO LONG-RANGE CONFORMATIONAL CHANGE IN THE FREE ENZYME
Experimental procedure
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 105.100, 105.100, 150.300 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | ? - 2.500 |
| R-factor | 0.169 * |
| Rwork | 0.169 |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | |
| Total number of observations | 156365 * |
| Number of reflections | 21162 * |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 5.4 * | took J. H. Hurley et al.,from original paper * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 28 (mg/ml) | |
| 2 | 1 | 1 | ammonium sulfate | 30 (%sat) | |
| 3 | 1 | 1 | 100 (mM) | ||
| 4 | 1 | 1 | 35 (mM) | ||
| 5 | 1 | 1 | citric acid | 9 (mM) | |
| 6 | 1 | 1 | dithiothreitol | 0.2 (mM) |
