4IC5
Crystal structure of Deg5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-04-08 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.090, 125.952, 83.325 |
Unit cell angles | 90.00, 102.91, 90.00 |
Refinement procedure
Resolution | 49.883 - 2.607 |
R-factor | 0.1844 |
Rwork | 0.182 |
R-free | 0.23210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l1j |
RMSD bond length | 0.007 |
RMSD bond angle | 1.026 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASES |
Refinement software | PHENIX ((phenix.refine: 1.7.1_743)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.600 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 33338 | |
Completeness [%] | 99.2 | 99.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 289 | 0.2M Calcium chloride dihydrate, 0.1M Tris-HCl, 25%(v/v) PEG400, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 289K |