4IBS
Human p53 core domain with hot spot mutation R273H (form I)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH3R |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-06-06 |
Detector | RIGAKU RAXIS IV++ |
Wavelength(s) | 1.54178 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.852, 70.179, 83.730 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.050 - 1.780 |
R-factor | 0.1642 |
Rwork | 0.163 |
R-free | 0.18070 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tsr |
RMSD bond length | 0.004 |
RMSD bond angle | 0.877 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1218) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 32.000 | 32.000 | 1.810 |
High resolution limit [Å] | 1.780 | 4.830 | 1.780 |
Rmerge | 0.059 | 0.034 | 0.335 |
Number of reflections | 74257 | ||
<I/σ(I)> | 16.6 | ||
Completeness [%] | 97.1 | 99.5 | 83.1 |
Redundancy | 7.3 | 7.5 | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 12% PEG 3350, 0.12M Mg-Formate, pH 6.1, Vapor diffusion, hanging drop, temperature 293K |