4IBQ
Human p53 core domain with hot spot mutation R273C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-04-27 |
Detector | ADSC QUANTUM 4r |
Wavelength(s) | 0.9310 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 68.731, 69.719, 83.510 |
Unit cell angles | 90.00, 90.10, 90.00 |
Refinement procedure
Resolution | 29.143 - 1.800 |
R-factor | 0.1349 |
Rwork | 0.134 |
R-free | 0.16310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tsr |
RMSD bond length | 0.006 |
RMSD bond angle | 0.987 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (dev_1223) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 30.000 | 30.000 | 1.830 |
High resolution limit [Å] | 1.800 | 4.880 | 1.800 |
Rmerge | 0.067 | 0.037 | 0.328 |
Number of reflections | 71943 | ||
<I/σ(I)> | 12.4 | ||
Completeness [%] | 97.9 | 97.7 | 96.9 |
Redundancy | 6.5 | 6.2 | 6.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.1 | 293 | 20% PEG 3350, 0.1M K-Acetate, 0.1M KF, pH 6.1, Vapor diffusion, hanging drop, temperature 293K |