4IBC
Ebola virus VP35 bound to small molecule
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 18-ID |
| Synchrotron site | APS |
| Beamline | 18-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 0.9793378 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 51.947, 65.902, 72.040 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 32.951 - 1.745 |
| R-factor | 0.1938 |
| Rwork | 0.191 |
| R-free | 0.24990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.025 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.8.1_1168) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.770 |
| High resolution limit [Å] | 1.740 | 4.720 | 1.740 |
| Rmerge | 0.125 | 0.047 | 0.696 |
| Number of reflections | 25426 | ||
| <I/σ(I)> | 7.5 | ||
| Completeness [%] | 98.7 | 93.8 | 90.3 |
| Redundancy | 6.4 | 6 | 3.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100 mM magnesium acetate, pH7.5, 15% PEG3350, 10% DMSO, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
