4IB8
Bovine beta-lactoglobulin (isoform A) in complex with dodecyl sulphate (SDS)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | OTHER |
| Temperature [K] | 110 |
| Detector technology | CCD |
| Collection date | 2012-05-09 |
| Detector | AGILENT ATLAS CCD |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 53.250, 53.250, 110.880 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 14.810 - 2.300 |
| R-factor | 0.22351 |
| Rwork | 0.217 |
| R-free | 0.28519 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bsy |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.405 |
| Data reduction software | CrysalisPro |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 14.810 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.054 | 0.257 |
| Number of reflections | 8404 | |
| <I/σ(I)> | 10.8 | 2.2 |
| Completeness [%] | 98.6 | 97.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 1.34M TRISODIUM CITRATE, 0.1M TRIS-HCL BUFFER, PH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
