4IAU
Atomic resolution structure of Geodin, a beta-gamma crystallin from Geodia cydonium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 5.2R |
| Synchrotron site | ELETTRA |
| Beamline | 5.2R |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-03-03 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 24.170, 61.302, 50.175 |
| Unit cell angles | 90.00, 95.45, 90.00 |
Refinement procedure
| Resolution | 20.000 - 0.990 |
| R-factor | 0.106 |
| Rwork | 0.106 |
| R-free | 0.12900 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHELXS |
| Refinement software | SHELX |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 20.000 |
| High resolution limit [Å] | 0.990 |
| Rmerge | 0.064 |
| Number of reflections | 80711 |
| Completeness [%] | 99.6 |
| Redundancy | 8.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 0.2M ammonium sulphate, 17 % w/v PEG 8000, 2mM dithiotreitol, 50mM Tris-HCl (pH 7.6) , VAPOR DIFFUSION, SITTING DROP, temperature 277K |
