4IAG
Crystal structure of ZbmA, the zorbamycin binding protein from Streptomyces flavoviridis
Experimental procedure
Experimental method | SAD |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-BM |
Synchrotron site | APS |
Beamline | 19-BM |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2012-08-03 |
Detector | ADSC QUANTUM 210r |
Wavelength(s) | 0.97929 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 38.787, 38.787, 268.524 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.590 - 1.900 |
R-factor | 0.1913 |
Rwork | 0.189 |
R-free | 0.23140 |
Structure solution method | SAD |
RMSD bond length | 0.016 |
RMSD bond angle | 1.836 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | MLPHARE |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 5.160 | 1.900 |
Rmerge | 0.068 | 0.057 | 0.434 |
Number of reflections | 10484 | ||
<I/σ(I)> | 11.2 | ||
Completeness [%] | 98.5 | 97.1 | 87.5 |
Redundancy | 9.7 | 8.9 | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4 | 289 | protein subject to reductive isopropylation, 0.8M Ammonium Sulfate, 0.1M Sodium Sitrate: HCl pH 4, VAPOR DIFFUSION, SITTING DROP, temperature 289K |