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4IAD

Low temperature X-ray Structure OF cAMP dependent protein kinase A in complex with high Mg2+ concentration, ADP and phosphorylated peptide pSP20

Experimental procedure
Experimental methodSINGLE WAVELENGTH
Source typeROTATING ANODE
Source detailsRIGAKU MICROMAX-007 HF
Temperature [K]100
Detector technologyIMAGE PLATE
Collection date2012-02-02
DetectorRIGAKU RAXIS IV++
Wavelength(s)1.54
Spacegroup nameP 21 21 21
Unit cell lengths57.522, 79.535, 97.914
Unit cell angles90.00, 90.00, 90.00
Refinement procedure
Resolution20.000 - 1.900
R-factor0.1894
Rwork0.186
R-free0.24350
Structure solution methodMOLECULAR REPLACEMENT
RMSD bond length0.012
RMSD bond angle1.340
Data reduction softwareHKL-3000
Data scaling softwareHKL-3000
Phasing softwareCNS
Refinement softwareCNS
Data quality characteristics
 Overall
Low resolution limit [Å]40.000
High resolution limit [Å]1.900
Number of reflections31508
Completeness [%]91.0
Crystallization Conditions
crystal IDmethodpHtemperaturedetails
1VAPOR DIFFUSION, SITTING DROP6.5277100 mM MES pH 6.5, 5 mM DTT, 15-20% PEG 4000, VAPOR DIFFUSION, SITTING DROP, temperature 277K

222036

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