4I9K

Crystal structure of symmetric W-W-W ClpX Hexamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-01
Detector	ADSC QUANTUM 315
Wavelength(s)	0.979
Spacegroup name	P 63
Unit cell lengths	119.430, 119.430, 111.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.150 - 5.000
R-factor	0.3196
Rwork	0.316
R-free	0.35440
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3hws
RMSD bond length	0.002
RMSD bond angle	0.468
Data reduction software	HKL-2000
Data scaling software	SCALA (3.3.16)
Phasing software	PHASER
Refinement software	PHENIX (1.5_2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.715	59.715	4.750
High resolution limit [Å]	4.506	14.250	4.506
Rmerge		0.144	0.272
Total number of observations		1019	5062
Number of reflections	5160
<I/σ(I)>	5.5	2.6	2.1
Completeness [%]	94.6	91.9	95.7
Redundancy	6.4	5.9	6.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	298	2 M ammonium sulfate, 0.15 M potassium sulfate, 4 mM ATP, 4 mM magnesium sulfate, 50 mM EDTA, pH 7.5, VAPOR DIFFUSION, temperature 298K