4I96
Crystal structure of the N-terminal two domains of the skeletal muscle ryanodine receptor (rabbit RyR1) residues 217-536
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Detector technology | CCD |
Collection date | 2010-05-10 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.97949 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 68.449, 68.449, 131.895 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 44.090 - 2.730 |
R-factor | 0.22462 |
Rwork | 0.224 |
R-free | 0.24132 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xoa |
RMSD bond length | 0.011 |
RMSD bond angle | 1.314 |
Data reduction software | xia2 |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 65.950 | 3.260 | 2.800 |
High resolution limit [Å] | 2.730 | 3.150 | 2.730 |
Number of reflections | 10067 | ||
Completeness [%] | 100.0 | 100 | 100 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8 | pH 8.0, EVAPORATION |