4I8X

Crystal structure of rabbit LDHA in complex with AP27460

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2008-12-05
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.54
Spacegroup name	P 1 21 1
Unit cell lengths	84.070, 139.850, 139.170
Unit cell angles	90.00, 94.48, 90.00

Refinement procedure

Resolution	50.000 - 2.230
Rwork	0.201
R-free	0.23580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i10
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	AMoRE
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000
High resolution limit [Å]	2.230	2.230
Number of reflections	146519
Completeness [%]	94.0

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	277	0.1 M Tris, pH 8.0, 30% PEG550 MME, VAPOR DIFFUSION, HANGING DROP, temperature 277K