4I8P

Crystal structure of aminoaldehyde dehydrogenase 1a from Zea mays (ZmAMADH1a)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2011-06-09
Detector	PSI PILATUS 6M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	181.190, 49.890, 154.530
Unit cell angles	90.00, 124.15, 90.00

Refinement procedure

Resolution	28.550 - 1.950
R-factor	0.1781
Rwork	0.176
R-free	0.21000
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3iwj
RMSD bond length	0.010
RMSD bond angle	1.070
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	BUSTER (2.10.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	2.070
High resolution limit [Å]	1.950	1.950
Number of reflections	83412
Completeness [%]	99.1	98.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	PEG 4K, 0.1M HEPES, 10% isopropanol, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K