4I81
Crystal Structure of ATPgS bound ClpX Hexamer
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-07-01 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 57.935, 199.167, 211.891 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 41.407 - 3.818 |
R-factor | 0.2719 |
Rwork | 0.271 |
R-free | 0.29470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3hws |
RMSD bond length | 0.006 |
RMSD bond angle | 0.783 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.5_2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.870 |
High resolution limit [Å] | 3.800 | 10.290 | 3.800 |
Rmerge | 0.074 | 0.032 | 0.843 |
Number of reflections | 24413 | ||
<I/σ(I)> | 7.8 | ||
Completeness [%] | 98.8 | 97.7 | 95.5 |
Redundancy | 6.7 | 6.2 | 6.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 4.8 | 298 | 75 mM sodium acetate, 1.9 M ammonium sulfate, pH 4.8, VAPOR DIFFUSION, temperature 298K |