4I7I
Crystal structure of rabbit ryanodine receptor 1 (residues 1-536) disease mutant L14R
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Detector technology | CCD |
Collection date | 2010-09-08 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97949 |
Spacegroup name | H 3 2 |
Unit cell lengths | 171.402, 171.402, 300.477 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 46.700 - 2.900 |
R-factor | 0.22885 |
Rwork | 0.228 |
R-free | 0.24869 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2xoa |
RMSD bond length | 0.006 |
RMSD bond angle | 1.013 |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.0) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.480 | 4.590 | 3.060 |
High resolution limit [Å] | 2.900 | 4.100 | 2.900 |
Number of reflections | 37869 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 22.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 8 | pH 8.0, EVAPORATION |