4I6Z

Crystal structure of the transcriptional regulator TM1030 with 24bp DNA oligonucleotide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2007-11-02
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97935
Spacegroup name	C 1 2 1
Unit cell lengths	187.008, 45.954, 82.510
Unit cell angles	90.00, 105.60, 90.00

Refinement procedure

Resolution	33.230 - 3.200
R-factor	0.2033
Rwork	0.200
R-free	0.26750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2id6
RMSD bond length	0.013
RMSD bond angle	1.651
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	MOLREP
Refinement software	REFMAC

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.260
High resolution limit [Å]	3.200	8.670	3.200
Rmerge	0.095	0.053	0.519
Number of reflections	11502
<I/σ(I)>	9.8
Completeness [%]	100.0	99.4	100
Redundancy	4.1	3.8	4.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	8	310	0.1M Tris, 20% PEG 4000, pH 8.0, VAPOR DIFFUSION, temperature 310K